Enzymatic synthesis of nucleoside S-mono and -triphosphates

Recently we have reported on the chemical synthesis of dNTP(3’F) and NTP(3’F) and their use as terminators of the biosynthesis of DNA and RNA, respectively [l-4]. During the course of the chemical synthesis of fluorodeoxyriboand 2 ’ -deoxyribo-NMP and NTP, we have pointed out that the isolated yields of pure compounds are varied within the broad limits depending on the structure of a heterocyclic base and/or a carbohydrate moiety (cf. [5]), and from assay to assay as well. On the other hand, it has been illustrated that various enzymatic methods are more effective for the nucleosides transformation to NMP as compared to chemical ones (detailed discussion see [6]). Of particular interest is the use of enzymatic methods for the transformation of NMP to NTP (see e.g. the data in 17-91). The -effectiveness of the enzymatic methods of phosphorylation depends on the specificity of pertinent enzymes that is of particular importance in the case of modified nucleosides and NMP [6,7]. The present paper is concerned with the investigation of the